EFFECT DIFFERENCE DOSES OF sGnRH-a + DOMPERIDON HORMONE ON SEMEN VOLUME, QUALITY OF SPERMATOZOA AND FRY HARD LIPPED BARB (Osteochilus hasselti CV)

Abstract

The research was conducted on April-May 2015 at Fish Hatchery and
Breeding Laboratory, Faculty Fisheries and Marine Science, University of Riau.
The purpose of this research to known the best effect injection difference doses of
sGnRH-a + domperidon hormone on semen volume, quality of spermatozoa and
fry hard lipped barb. This research used experimental method with Completely
Randomized Design (CRD) five treatments and three replications. The treatments
with injection doses sGnRH-a + domperidon: control (2 ml NaCl physiology 0,9
%/kg body weight), P1 (0,3 ml sGnRH-a + domperidon /kg body weight), P2 (0,4
ml sGnRH-a + domperidon /kg body weight), P3 (0,5 ml sGnRH-a + domperidon
/kg body weight), P4 (0,6 ml sGnRH-a + domperidon /kg body weight). The result
showed the best effect injection dose is 0,5 ml sGnRH-a + domperidon/kg body
weight could generate semen volume 0,633 ml, spermatozoa concentrate 1404 x
107 cell/ml, motility 71,33%, viability 86,54%. Meanwhile, spawning result with
best effect injection showed fertility rate 81,33%, hatching rate 75,66%, survival
rate of fry 95,1%, growth weight absolute 0,278 g, growth length absolute 1,919
cm and growth daily rate 10,17%
Key word: Dose sGnRH-a + domperidon, semen volume, quality of
spermatozoa, fry, hard lipped barb (Osteochilus hasselti CV)