ANALISIS TOTAL FENOLIK, FLAVONOID, DAN AKTIVITAS ANTIOKSIDAN PADA DAUN SERTA BATANG TANAMAN Coleus amboinicus Lour (BANGUN-BANGUN)

Abstract

The objective of this study was to analyze the total phenolics, flavonoids, and
antioxidant activity of leaves and stems of C. amboinicus. Two different watering
treatments (EM5 and FPE) were applied to C. amboinicus during cultivation. The
results showed that leaves of C. amboinicus treated with FPE had the highest total
phenolic and flavonoid content than other samples. The total phenolic content was
2.09 mg Gallic Acid Equivalent (GAE)/g of dry weight (DW) and the total flavonoid
content was 6.218 mg Catechin Equivalent (CTE)/g of DW respectively. The leaves
treated with FPE also had the highest antioxidant activity by FRAP test (0.058 mmol
Fe +2 /g DW). The highest antioxidant activity by DPPH test was found in the leaves
treated with EM5 (1.42 mg Ascorbic Acid Equivalent (AAE)/g DW). From this study it
can be concluded that leaves of C. amboinicus was the best source of antioxidant. FPE
watering treatment gave a better influence on total phenolic, flavonoid, and antioxidant
activity of C. amboinicus than EM5.