IDENTIFIKASI DAN UJI TOKSISITAS EKSTRAK METANOL DARI DAUN TANAMAN SIRSAK (Annona muricata L)
Abstract
Soursoup (Annona muricata L) was used in herbal medicine. This Annonaceae family
member contains alkaloids, tannins, and several other chemical constituents, including
asetogenin that allegedly to have cytotoxic potential. The n-hexane and methanol
macerating method was applied to isolate the secondary metabolites from the stem bark
of this plant. The separation was carried out by vacuum liquid chromatography (VLC),
column chromatography, and gel chromatography respectively. Characterization of the
fractions was established using UV-Vis and FTIR spectrophotometry. Toxicity assay
was conducted by Brine Shrimp Lethality Test (BSLT) method. The LC 50 of 3 rd to 11 th
fractionts of the methanol extract wer 4481.25; 37; 11.46; 0.85; 3.02; 0.23; 10.97; 8.53
and 4093 ppm, respectively. The 3 rd and 11 th fractionts were not toxic, whereas other
fractions were toxic and potential to be used as an anticancer.
member contains alkaloids, tannins, and several other chemical constituents, including
asetogenin that allegedly to have cytotoxic potential. The n-hexane and methanol
macerating method was applied to isolate the secondary metabolites from the stem bark
of this plant. The separation was carried out by vacuum liquid chromatography (VLC),
column chromatography, and gel chromatography respectively. Characterization of the
fractions was established using UV-Vis and FTIR spectrophotometry. Toxicity assay
was conducted by Brine Shrimp Lethality Test (BSLT) method. The LC 50 of 3 rd to 11 th
fractionts of the methanol extract wer 4481.25; 37; 11.46; 0.85; 3.02; 0.23; 10.97; 8.53
and 4093 ppm, respectively. The 3 rd and 11 th fractionts were not toxic, whereas other
fractions were toxic and potential to be used as an anticancer.
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