INDUKSI TUNAS IN VITRO DARI TUNAS BATANG (Sucker) TANAMAN NANAS (Ananas comosus (L.) Merr.) ASAL KAMPAR DENGAN PENAMBAHAN 6-BENZYLAMINOPURINE (BAP)

Abstract

Pineapple (Ananas comosus (L.) Merr.) is a fruit plant that is liked most people. Fruit
production and seedling availability have to be balanced in order to provide consumer’s
need. This problem can be solved using an in vitro technique. This research used
Group Randomized Design (GRD) Factorial with two factors which are concentration
of BAP and explant section. The concentrations of BAP used in this study were 0,0;
0,5; 1,0; 1,5; 2,0; 2,5 mg/l on MS medium. Explant source was collected from sucker,
with two different parts whole bud and bud sectioned longitudinally. The objectives of
this research were to get the optimal concentration, the best explant's cut part, and to get
the best treatment interaction and combination for in vitro shoot induction. The result
showed that the whole bud that could give response to BAP was indicated by shoot
formation. The shoot was formed in the percentage of 25% with concentration 1,0 mg/l
on 12 th day.