ISOLASI DAN SELEKSI JAMUR SELULOLITIK DARI HUTAN ARBORETUM UNIVERSITAS RIAU

Abstract

The Arboretum University of Riau constitutes of a secondary forest in Pekanbaru. Many
plant genera exist in this Arboretum, therefore the litter produced has high content of
cellulosa that may provide suitable environment for many cellulolitic fungus. The
purpose of this study was to obtain the cellulolytic fungus isolates by screening the soil
that collected from Arboretum University Riau, using Potato Dextrose Agar medium.
The selection of cellulolytic fungus used Carboxyl Methyl Cellulose medium and was
indicated by the appearance of clear zona. Cellulase activities were determinated using
the ratio of clear zone and colony diameter. The result identified 53 cellulolytic fungus
isolates. The L4H4 isolate had the highest ratio (3,51). There were three ratio criterions
of clear zone to colony diameter in this study, i.e. high (24,53%), medium (35,85%) and
low (39,62%). The characterization result showed that 15 fungus isolates belonged to
the genera Aspergillus, Penicillium, Paecilomyces, Mucor and 2 isolates were
unidentified.