INDUKSI TUNAS IN VITRO DARI EKSPLAN TUNAS BUAH (SLIP) TANAMAN NANAS (Ananas comosus (L.) Merr.) ASAL KAMPAR DENGAN PENAMBAHAN 6-BENZYLAMINOPURINE (BAP)

Abstract

Pineapple (Ananas comosus (L.) Merr.) is widely cultivated. One of pineapple plantation
centers in Riau Province is the Kampar district. The vegetative seedling technique of
pineapple is done using tissue culture techniques. Pineapple plant part that used as explants
in this study is fruit buds. Explants were cut into whole bud and bud sectioned
longitudinally. The growth regulator used was BAP with different concentration, i.e. 0.0,
0.5, 1.0, 1.5, 2.0, 2.5 mg/l in Murashige and Skoog (MS) medium. This study aims to
determine the optimum concentration of BAP as well as the best explant part and to get the
best combination between treatments in shoot induction. The study design used randomized
block design (RBD) factorial. The concentration of 1.0 mg/l BAP on whole bud explants
induced 3 buds, while the bud sectioned longitudinally induced only one shoot on control,
and concentration at 0.5 and 1.0 mg/l. Whole bud explants induced shoots in all of the
treatments except the control. The interaction between treatment and the combination that
could induced the greatest number of shoot was the whole bud explants with the addition
of 1.0 mg/l BAP.