ISOLASI DAN KARAKTERISASI FUNGI ENDOFIT DARI KULIT BUAH MANGGIS (Garcinia mangostana L) SEBAGAI ANTIMIKROBA TERHADAP Candida albicans, Staphylococcus aureus DAN Escherichia coli

Abstract

Garcinia mangostana Linn is one of the popular local plants that has been used as
medicinal herb. Extract of Garcinia mangostana has been reported having anti-
inflammatory, anti-tumour, anti-oxidant and antimicrobial activities. Bioactive
compounds from extract of mangosteen fruit husk were thought to produce by
endophytic fungi. The purpose of this research were to isolate and characterize
endophytic fungi from mangosteen fruit husk that potential to produce antimicrobial
compound. Antimicrobial activity was determined by measuring the inhibition zone
with Agar Disc and Kirby-Bauer assay methods using pathogenic microbes i.e. Candida
albicans, Staphylococcus aureus and Escherichia coli. Eleven of twenty isolates
endophytic fungi which were obtained from mangosteen fruit husk were known to have
antimicrobial activities. Endophytic fungi producing antimicrobial belongs to genus
Aspergillus, Penicillium, Trichoderma, Alternaria and Fusarium. Endophytic fungi
having the highest antimicrobial activities in agar disc assay were Penicillium
sp.1KMA, Aspergillus sp.5KMR, Aspergillus sp.1KMA, Fusarium sp.KMR and
Aspergillus sp.3KMB. Endophytic fungi having the highest antimicrobial activities in
Kirby-Bauer assay was Penicillium sp.1KMA. Antimicrobial activities of Penicillium
sp.1KMA was significantly different with the other four endophytic fungi.